Manufacturing method of medical sterilized isotonic solution having low-concentratedly controlled free chlorine including hypochlorous acid therein

ABSTRACT

The present invention relates to a manufacturing method of medical sterilized normal saline, more specifically, to such a method for manufacturing sterilized normal saline for medical purpose with effective sterilizing efficacy comprising: a step of disposing at least one electrode set immersed in saline solution of pH 4.0 to pH 7.5 including a pair of electrodes with flat surface separated from each other by an interval between 1 mm and 3 mm, the flat surfaces of the electrodes facing each other; and a step of supplying 3 OmA to 20 OmA direct current to the electrodes by applying 2.4V to 3.3V DC power to the electrodes; wherein free chlorine is reliably and stably generated as having concentration range between 0.17 ppm and 6 ppm from electrolysis between electrodes.

TECHNICAL FIELD

The present invention relates to a manufacturing method, moreparticularly, to such a method reliably manufacturing low-concentratedlycontrolled free chlorine including hypochlorous acid (HOCl) thereinusing electrolysis in order to disinfect various germs causing diseases.

BACKGROUND ART

As is well known, environmental diseases have been increased as air andsoil has been polluted, and the concern for a well-being and health hasbeen increased. Accordingly, rhinitis' patients washing their noses withnormal saline in the market have been increased.

Hypochlorous acid (HOCl) is well known as effective and harmlesschemicals to people as well as chemicals for disinfecting variousviruses. However, Journal of Burns and Wounds, a medical journalpublished on Apr. 11, 2007, showed in its article titled “HypochlorousAcid as a Potential Wound Care Agent” that HOCl has never been used asmedical supplies for curing viral infection.

On the other hand, normal saline is achieved by four chemical mechanismsas follows.

Cl₂+H₂O

HOCl+H⁺+Cl⁻  Chemical equation 1

Cl₂+H₂O

HOCl+H⁺+Cl⁻  Chemical equation 2a

Cl₂+H₂O

HOCl+H⁺+Cl⁻  Chemical equation 2b

OCl⁻+H⁺

HOCl  Chemical equation 3

That is, HOCl can be generated by hydrolyzing chlorine gas in accordancewith the chemical equation 1, or by electrolyzing saline solution inaccordance with the chemical equation 2a and 2b, or by oxidizinghypo-chlorite in accordance with the chemical equation 3. In the case ofchemical equation 1, however, it is troublesome and risky to handlechlorine gas which is sometimes in the form of poisonous gas. Also, withregard to the chemical equations 2a and 2b, it is written in the abovearticle that it is very difficult to realize the targeted concentrationof HOCl by electrolysis. Therefore, the above journal shows that it ismost desirable to generate HOCl for medical uses through usingcommercially available hypo-chlorite in accordance with the chemicalequation 3.

On the other hand, it is written at 71 page of the above journal thatthe minimum bactericidal concentrations (MBC) of HOCl enough todisinfect viruses at normal temperature are shown as follows.

TABLE 1 Germs MBC (ppm) Escherichia coli 0.7 Pseudomonas aeruginosa 0.35Staphylococcus aureus 0.173 Staphylococcus epidermidis 0.338 Micrococcusluteus 2.77 Corynebacterium amycolatum 0.169 Haemophilus influenzae0.338 Proteus mirabilis 0.340 Staphylococcus hominis 1.4 Staphylococcushaemolyticus 0.338 Staphylococcus saprophyticus 0.35 Candida albicans2.7 Klebsiella pneumoniae 1.7 Serratia marcescens 0.169 Sterptococcuspyogenes 0.169 Enterobacter aerogenes 0.676 Candida albicans 0.17Methicillin-resistent Staphylococcus aureus 0.682 Vancomycin-resistentEnterococcus faecium 2.73

From the above table 1, even very low concentration of HOCl cansterilize most of germs effectively. However, a solution withunlimitedly higher concentration of HOCl would not be used for medicaluses. Therefore, the above journal describes that the unreliable amountof HOCl generated by an electrolysis cannot be used for medically curingany infection such as by directly injecting or spraying it into a humanbody. Specifically, when the concentration of HOCl exceeds over 6 ppm,it causes trouble on sensitive parts of human body such as a mucosa of anose and eyes, further it causes the stench of the solution, and thus, asolution with over 6 ppm concentration of HOCl has been regarded as notbeing used for medical treatment. In this regard, the document publishedon January in 1994 by the U.S. Environmental Protection Agency has alsoannounced that a desirable concentration of the free chlorine (in otherwords, it is referred as ‘residual chlorine’) for an adult with weightof 70 Kgf is recommended as less than 6 ppm.

Therefore, it is most important to manufacture sterilized normal salinewith maintaining the precise low concentration of free chlorineincluding HOCl in order for applying HOCl to a medical treatment. Also,maximizing the ratio of HOCl in the free chlorine is needed for bettersterilizing effect.

On the other hand, HOCl has a relatively long half-life time of 44 hourin the inside of a human body. However, in the water, as shown in FIG.16, the half-life time of HOCl falls to 3 minutes, and thus, theeffective sterilizing efficacy of HOCl cannot be expected after thehalf-life time is passed.

Considering above, HOCl has been widely admitted for its possible usefor medical treatments because HOCl has a sterilizing efficacy againstdiverse germs. However, as HOCl has very short half-life time outside ofhuman body, and as HOCl has not been realistically manufactured withbeing controlled within the predetermined lower range of concentration,it has been regarded as impossible to apply HOCl to patients for medicalpurpose such as treating diseases infected by diverse germs.

Accordingly, there has been great need for method of manufacturing HOClwith the predetermined constant lower range of concentration enough toterminate germs as well as not to cause the disgusted feeling to patentsso that the free chlorine especially including HOCl can be realisticallyapplied to patents for medical purpose.

DISCLOSURE OF INVENTION Technical Problem

These disadvantages of the prior art are overcome by the presentinvention. It is an object of the present invention to provide a methodfor sterilized normal saline for medical purpose with the constantly andreliably controlled as low concentration between 0.17 ppm and 6 ppm sothat the sterilized normal saline has effective sterilizing efficacywithout causing patients to feel disgusted.

Another object of the present invention is to provide a method forsterilized normal saline for medical purpose with the regularlycontrolled concentration of free chlorine which is inodorous so that itdoes not make trouble on diseases such as a mucosa of a lung, eyes, anose or a skin and therefore can be comfortably used for sensitivepatients.

Still another object of the present invention is to provide a portableapparatus for manufacturing sterilized normal saline for medical purposeso that patients can use the sterilized normal saline for theirtreatment immediately after manufacturing, which includes HOCl having ashort half-life time outside of human body.

Therefore, users can manufacture the sterilized normal saline with lowlycontrolled concentration of HOCl everywhere they want so that themanufactured normal saline can be applied to various parts such as aninflammation, an athlete's foot, atopic dermatitis, etc.

Yet another object of the present invention is to provide a portableapparatus for manufacturing normal saline having controlled lowlyconcentrated HOCl generated from the electrolysis by applying lowelectric current and low voltage so that the life time of batteries canbe prolonged.

Still, another object of the present invention is to provide a methodfor manufacturing sterilized normal saline by electrolysis with usingsubacid or neutral water of pH 4.0 to pH 7.5 in order to raise the ratioof HOCl with strong sterilizing efficacy.

Technical Solution

In order to attain the above mentioned object, the present inventionprovides a method for manufacturing sterilized normal saline for medicalpurpose with effective sterilizing efficacy comprising: a step ofdisposing at least one electrode set immersed in saline solution of pH4.0 to pH 7.5 including a pair of positive electrode and negativeelectrode with flat surface separated from each other by an intervalbetween 1 mm and 3 mm, the flat surfaces of the electrodes facing eachother; a step of supplying 30 mA to 200 mA direct current to theelectrodes by applying 2.4V to 3.3V DC power to the electrodes; whereinfree chlorine is generated as having concentration range between 0.17ppm and 6 ppm from electrolysis between electrodes.

As the medical normal saline of the present invention is manufactured byuse of subacid or neutral normal saline of pH 4.0 to pH 7.5, as shown inFIG. 13, the ratio of HOCl in the solution having experienced theelectrolysis can be maximized at least 50% or up to 100% thereof,thereby achieving high treatment efficacy even with the small amount ofthe free chlorine and also preventing the acid degree of the solutionfrom causing patients to feel troublesome at their mucosa of nose oreyes. Further, the medical normal saline of the present invention can bemanufactured by mixing salt and tap water or underground water ofsubacid or neutral pH which can be easily obtained.

Herein, the solution can have its up to 3% high salt concentrationdifferent from the normal saline so that the solution of highconcentration may be supplied to insensitive organs of human body whichare not sensitive.

When the gap of the pair of the electrodes is less than 1 mm, thecurrent between the electrodes is excessively raised, and gasesgenerated during the electrolysis cannot get out of the inner spacebetween the electrodes, and thus, the free chlorine is sometimesgenerated too much or is sometimes generated too little. That is, theamount of the free chlorine generated thereduring is not consistent whenthe gap of the electrodes is less than 1 mm. When the gap of the pair ofthe electrodes is over 3 mm, high electric current is required to flowbetween the pair of electrodes. However, when the high electric currentis supplied to the pair of the electrodes, the current becomesexcessively increased as shown in FIG. 14 and thus it becomes impossibleto make low current flow between the electrodes so that the controlledsmall amount of the free chlorine cannot be generated, and also, theconsumption of the electric power becomes too much so that it cannot beapplied to a portable device. That is, in order that the controlled lowconcentrated free chlorine can be reliably generated by theelectrolysis, it is required to constantly maintain the current and theamount of electric charges moving across the electrodes to be very low.

With maintaining the gap between the electrodes, when the DC voltagebetween 2.4V and 3.3V is applied thereto, DC current between 30 mA and200 mA flows therebetween. When the DC current continues to flow betweenthe electrodes, solution with the controlled low concentration between0.17 ppm and 6.0 ppm of free chlorine is obtained. Herein, when DCvoltage less than 2.4V is applied to the electrodes, as the voltagedifference cannot overcome the resistance of the normal saline and thusthe electric current cannot flow between the electrodes. When DC voltagemore than 3.3V is applied to the electrode, it becomes very difficult tomaintain the DC current within the constant range because the currentbetween the electrodes increases very sharply, and thus, theconcentration of the free chlorine also increases sharply, andaccordingly, it becomes very difficult to generate the controlled lowconcentrated free chlorine.

In this regards, the net current to an external circuit via oneelectrode is determined by the difference between the oxidation currentand the deoxidation current. Specifically, as shown in FIG. 14,according to Butler-Volmer equation, the net current increasesproportionally when the overvoltage is small while the net currentexponentially increases when the overvoltage is larger than apredetermined value. That is, the current flowing between the electrodesimmersed in the saline solution having concentration of 0.3% to 3%depends on the voltage applied thereto and the resistance between theelectrodes. Therefore, as saline solution between the electrodes havingthe simple flat surface respectively plays a role in an electricresistance, electric current will not flow when small voltage less thanDC 2.4V is applied to the electrodes. On the other hand, when largevoltage more than DC 3.3V is applied thereto, excessive large electriccurrent will flow therebetween and excessive amount of the free chlorineis generated within very short time and thus it becomes very difficultto control the concentration of the free chlorine to be lower range.

The normal saline having lowerly controlled concentration of theresidual chlorine can be reliably and stably manufactured by applying DClow current and low DC voltage. The normal saline manufactured by theabove method is not irritative and does not have sickening smell andthus can be used in the wide range of usage for medical purpose such aslung, eyes, nose and skins without causing patients to feel disgusted.

Herein, as normal saline manufactured by the present invention isneutral or subacid, the most part of the free chlorine generated duringthe electrolysis becomes HOCl having strong sterilizing efficacy. It isdesirable to preset the concentration of the free chlorine between 3 ppmand 4 ppm thereby minimizing the possibility that the concentration ofthe free chlorine exceeds 6 ppm.

The mechanism of manufacturing the medical normal saline with oxidantssuch as ozone (O₃), hydrogen peroxide (H₂O₂), OH−radicals, HOCl, OCl⁻ ofthe present invention is realized by the following (1) to (5)procedures.

(1) The process of ozone creation starts from electrolysis of H₂O andfinished with a combination of O and O₂.

*H₂O-->H⁺+(OH)_(ads) +e ⁻

(OH)_(ads)-->(O)_(ads)+H⁺ +e ⁻

2(OH)_(ads)-->O₂+2H⁺+2e ⁻

2(O)_(ads)-->O₂

(O)_(ads)+O₂-->O₃

(2) H₂O₂ is made by a direct process of electrolysis of O₂ and indirectprocess of a combination of OH radicals, a medium generated by O₃. Thatis,

direct course,

O₂ +e ⁻-->O₂ ⁻

O₂+2H⁺+2e ⁻-->H₂O₂

Indirect course,

OH.+OH.-->H₂O₂

(3) HOCl is formed by chemical reaction with H2O after combining withCl⁻ existing in water with Cl₂.

2Cl⁻-->Cl₂+2e ⁻

2H₂O+2e ⁻-->H₂+2OH⁻

*Cl₂+H₂O-->HOCl+H⁺Cl⁻

(4) OH radicals are created and vanished too soon to measure itdirectly, but in the case of ozone existing in water, OH radicals arefinally created forming radical chain cycle with reacting with HO₂ ⁻,conjugate base of H₂O₂, or OH⁻.

O₃+OH-->Radical Chain Reaction-->OH.

O₃+HO²⁻(conjugate base of H₂O₂-->Radical Chain Reaction-->OH.

(5) Microorganisms existing in water get removed or inactivated by theoxidants, the following microorganism is removed by electroadsorptionand the following microorganics gets removed by direct electrolysisreacting with e⁻.

That is, regarding the microorganism,

M(Microorganism)-->Electrosorption-->Inactivation

Also,

M(Microorganism)+O₃-->Inactivation

M+OH.-->Inactivation

M+HOCl-->Inactivation.

And, regarding microorganics,

M(Microorganics)+e⁻-->M−

Also,

M(Microorganics)+0-->Product

M+OH.-->Product

M+HOCl-->Product

That is, during electrolysis, oxidation or sterilization is activelyperformed by the various oxidants including the free chlorine such asHOCl, OCl⁻ formed in the (1) to (5) procedures. During and after theelectrolysis, germs are killed by the sterilizing efficacy of HOClcontained in the medical normal saline as the majority of the freechlorine is generated during the electrolysis.

Here, H₂O₂ generated in the procedure of electrolysis can make freeradicals, HO.+O. and these free radicals decompose proteins into peptideand amino acid with low molecular weight so that protein turns intowater-soluble substance and converges on a double-bonded area, andepoxide is formed. (For instance, C═C—R become C—C—R) More specifically,free radicals formed in H₂O₂ have high reactivity and attacks otherorganic molecules like protein for stability of itself herebyoxidization of H₂O₂ decomposes protein into amino acid, water-solublesubstance and remove protein, one of causes for allergies.

That is, the oxidants including the free chlorine generated during theelectrolysis of the present invention has a strong efficacy of killinggerms, fungi, bacteria and viruses as well as an efficacy of decomposingthe double-bonded area between carbon molecules and nitrogen moleculesthereby removing proteins to cause allergy and atopy dermatitis.Therefore, the medial normal saline of the present invention can beapplied to treatment of allergy or atopy dermatitis. Further, themedical normal saline of the present invention can be used for curingrhinitis and atopy by transforming the protein to provoke an allergy.Also, the free chlorines such as HOCl are effective at curing HPV (humanpapillomavirus) to provoke uterine cancer, and thus can be supplied intothe womb.

On the other hand, the inventor of the present invention newly foundthat the concentration of the free chlorine can be precisely controlledby converting the direction of the electric current between theelectrodes during the electrolysis. Specifically, in case that theelectric current is supplied to the electrodes without converting thedirection thereof during the electrolysis, the experiment shows that thefree chlorine is generated more rapidly and suddenly, and that theconcentration of the free chlorine becomes different with one another ashaving larger variations in accordance with each experiments comparedwith the cases with converting the direction of the electric current. Inorder to increase the effect of converting the DC current, the period ofconverting DC current is desired to be set 1 second to 20 seconds. Incase that the period is set over 20 seconds, the effect thereof becomesinsignificant. Also, considering that the procedure of manufacturing thesmall amount less than 100 ml of sterilized medical normal saline withthe free chlorines by supplying DC current to electrodes is realized for20 seconds to 60 seconds, it is effective that the period is not over 20seconds.

The method of the medical normal saline of the present invention can berealized just with a container for accommodating normal saline,electrodes in the container and a power supply to supply DC current tothe electrodes. Thus, the method of the present invention can beachieved by a light apparatus having only a few requisite components.Therefore, the method of the present invention can be realized as aportable apparatus to be used not only by professional medicalinstitutions but also by an individual patient, i.e., customer level.

Especially, HOCl generated in the medical sterilized normal saline isvery unstable in neutral or subacid area, and thus is tend to be reducedto a half only for 3 minutes. Therefore, the free chlorines should besupplied to patients within 3 minutes from manufacturing the medicalnormal saline. From this point of view, by realizing the method of thepresent invention by a portable apparatus, many patients or customerscan use the medical normal saline to to-be-cured region such as insideof nose, eyes, skins of allergy, teeth, teethridge, lung, throat,bronchi, womb, etc., until the free chlorines do not lose the most ofsterilizing efficacy, i.e., right after manufacturing the medical normalsaline for themselves. Once HOCl is supplied to the inside of mammalbodies, the half-life time of HOCl increases to 44 hours. Therefore,prompt supply into the body right after manufacturing the medical normalsaline can obtain enough time to sterilize or kill germs, fungi,bacteria, the causes of diseases.

On the other hand, the present invention provides a method formanufacturing sterilized normal saline for medical purpose withsterilizing efficacy comprising: a step of disposing at least oneelectrode set immersed in saline solution of pH 4.0 to pH 7.5 includinga pair of electrodes, each of which has a plurality of domains separatedfrom one another on the facing surface of the each electrode, theplurality of domains on the facing surface of the one electrode beingfaced to the plurality of domains respectively on the facing surface ofthe other electrode with an interval between 1 mm and 3 mm; a step ofsupplying 30 mA to 180 mA of DC current to the electrodes by applying2.2V to 3.2V DC voltage to the electrodes; and a step of converting thedirection of DC current between the electrodes at least one time,wherein the total area of the plural domains of each of the electrodescovers 4% to 25% of the total area of the facing surface of eachelectrode and the free chlorine is generated as having concentrationrange between 0.17 ppm to 6 ppm during the electrolysis.

That is, a plurality of domains are formed on the surface of theelectrodes which faces each other, and a plurality of current paths areformed between the plurality of domains, and therefore, constant andslight electrolysis occurs at the plurality of the domains. Accordingly,the constant chemical reactions for generating the free chlorines occurat the plurality of domains on a small scale over the whole surface ofeach of the electrodes, and thus it is much easier to precisely controlthe concentration of the free chlorine at precisely lowered level.

That is, in case that electrolysis occurs at the electrodes which has aplurality of small multi-points (i.e., domains) on their surfaces facingeach other and thus form a plurality of current paths between themulti-points, compared with the case that the electrolysis occurs at theelectrodes only having simple flat surfaces facing each other, it wasfound that the more and smaller bubbles are generated with uniformlydistributed during the electrolysis.

Especially, in the case that the multi-points of domains are formed onthe electrodes, as electric charges are concentrated and flow along thepaths between the multi-points of domains, the electric current can flowbetween the electrodes with the lowerly supplied voltage of 2.2V. orwith the lower electric current, and the consumption of the electricpower can be lowered, and thus the life of battery can be prolonged fora longer time. Most of all, instead of supplying lots of electriccharges at the multi-point of domains at a time, electric charges aredesired to be constantly and continuously supplied to the multi-point ofdomains to induce the electrolysis, so that the chemical reactions areconstantly and uniformly realized because the reactants for generatingHOCl can be reacted without any hindrances. Accordingly, whenelectrolysis occurs with the electrodes having the multi-points ofdomains facing each other in the same condition, the free chlorines aremore constantly and slowly generated and thus it becomes easier tocontrol the generation rate of the free chlorines thereby enabling tocontrol and generate the free chlorines within targeted lowerconcentration thereof.

Similarly, during the supply of the DC voltage to the electrodes, byincluding the step of converting the direction of the electric currentbetween the electrodes, the concentration of the free chlorines can bemore precisely controlled to be lowered less than 6 ppm.

Herein, the electrode set comprises a plate-shaped positive electrodehaving a plurality of positive rods divided by a plurality of positiveslots in one direction which are parallel with one another; and aplate-shaped negative electrode having a plurality of negative rodsdivided by a plurality of negative slots in one direction which areparallel with one another wherein a plurality of domains are formed bythe areas overlapped in the perpendicular direction to the surface ofthe electrodes in condition that the positive electrode and the negativeelectrode are disposed in parallel, and that the positive rods are notdisposed to be parallel with the negative rods.

From this construction, small electric current flows between theplurality of domains which are formed by the areas overlapped in theperpendicular direction to the surface of the positive electrode and thenegative electrode, and the electrolysis constantly occurs at theuniformly distributed areas over the whole surface of the electrodesthereby enabling to precisely control the concentration of the freechlorines to the low level.

Herein, it is desirable that the positive rod and the negative rod arearrayed at right angle each other so that the distances among thedomains and the sizes of the domains can be constant. The width of thepositive rods is smaller than that of the positive slots while the widthof the negative rods is smaller than that of the negative slots.Therefore, the domains of the negative electrode are arrayed more apartfrom the other domains of the negative electrode than the size of thedomains. Further, the reactants required to generate the free chlorinesare more distributed and thus the reactants react uniformly over thesurface of the electrodes.

On the other hand, the electrodes include a plurality of positiveprojections protruded from the positive electrode; and a plurality ofnegative projections facing the each of the positive projections andprotruded from the negative electrode, wherein the plurality of domainsare formed on the top surface of the projections facing each other. Theprojections can be formed of cone shape or circular column shape orother form. In case that the domains are formed of the projections, thesame or similar effects to the described above can be obtained.

Herein, the conversion period for changing the direction of currentbetween the electrodes is desirable to be set between 1 second and 20seconds. Also, when the amount of the normal saline is 10 ml to 100 ml,the normal saline can be manufactured with a portable apparatus by acustomer level. At this case, the medical normal saline with 0.17 ppm to6 ppm concentration of the free chlorines can be obtained by supplyingDC current for 10 seconds to 60 seconds.

ADVANTAGEOUS EFFECTS

As described above, the present invention provides a method formanufacturing sterilized normal saline for medical purpose witheffective sterilizing efficacy comprising: a step of disposing at leastone electrode set immersed in saline solution of pH 4.0 to pH 7.5including a pair of positive electrode and negative electrode with flatsurface separated from each other by an interval between 1 mm and 3 mm,the flat surfaces of the electrodes facing each other; a step ofsupplying 30 mA to 200 mA direct current to the electrodes by applying2.4V to 3.3V DC power to the electrodes; wherein free chlorine isgenerated as having concentration range between 0.17 ppm and 6 ppm fromelectrolysis between electrodes, whereby the medical normal saline withlower concentration of the free chlorines can be stably and reliablyobtained.

Further, the present invention enables to apply the medical normalsaline to sensitive patients because the medical normal saline ismanufactured by strictly and precisely controlling the concentration ofthe free chlorines within the lower range, and thus does not have anystimulus on the sensitive part of body such as mucosa of lung, eye,nose, skin and does not have offensive smell owing to much of chlorine.

Also, the present invention enables patients or customers to manufacturethe medical normal saline by electrolysis for themselves at any time andat any place, which contains the precisely controlled concentration ofthe free chlorines, and thus, enables patients or customers to apply thefresh medical normal saline with more sterilizing efficacy for theirpurpose directly after manufacturing it, thereby maximizing the curingefficacy.

And, the present invention enables to use battery of a portableapparatus for manufacturing the medical normal saline for longer timebecause the electrolysis occurs by applying low voltage and low currentto the electrodes thereby reducing the consumption of the electricpower.

Also, the present invention uses subacid or neutral water of pH 4.0 topH 7.5 during the electrolysis so as to maximize the content of HOClhaving sterilizing efficacy of 80 times more than OCl⁻, and provides amedical normal saline having high sterilizing efficacy.

BRIEF DESCRIPTION OF THE DRAWINGS

Accordingly, the present invention will be understood best throughconsideration of, and reference to, the following Figures, viewed inconjunction with the Detailed Description of the Preferred Embodimentreferring thereto, in which like reference numbers throughout thevarious Figures designate like structure and in which:

FIG. 1 is a perspective view of an apparatus for realizing the medicalsterilizing normal saline in accordance with one embodiment of thepresent invention.

FIG. 2 is a exploded perspective view of FIG. 1

FIG. 3 is a partly sectional perspective view of FIG. 1

FIG. 4 is a perspective view of the electrodes of FIG. 1

FIG. 5 is a dissembled perspective view of FIG. 4.

FIG. 6 is a circuit view showing power supply to the electrodes of theapparatus of FIG. 4.

FIG. 7 is a flowchart for showing the operation principle of theapparatus of FIG. 1.

FIG. 8 is a cross-sectional view by cut-line VI-VI.

FIG. 9 is a front view of other construction of electrodes applicable tothe electrodes of FIG. 4.

FIG. 10 is an enlarged part view of the second drawing of FIG. 9.

FIG. 11 is a schematic projected view when the first drawing of FIG. 9is overlapped on the second drawing of FIG. 9.

FIG. 12 is a view of another construction of electrodes applicable tothe electrodes of FIG. 4.

FIG. 13 is a graph of the relation between the form of free chlorine andpH at 20° C. and 100 m/l

FIG. 14 is a graph of the relation of Butler-Volmer equation betweenovervoltage of electrodes and the current.

FIG. 15 is a view of a circuit for maintaining the current constantlybetween the electrodes in spite of the change of the salt concentrationor the change of the voltage applied to the electrodes.

FIG. 16 is a graph of the amount of the free chlorine in accordance withthe 20 seconds operation time.

FIG. 17 and FIG. 18 are the perspective view of cleaning module ofcontact lenses combined with the inlet of the container of FIG. 2.

FIG. 19 is a schematic diagram of reversed state of the apparatus formanufacturing the medical normal saline with the plug.

FIG. 20 is a detailed view of FIG. 19.

BEST MODE FOR CARRYING OUT THE INVENTION

The foregoing and other objects, features, aspects and advantages of thepresent invention will become more apparent from the following detaileddescription of the present invention when taken in conjunction with theaccompanying drawings.

In describing the present invention, detailed description of laid-outfunction or structure is omitted in order to clarify the gist of thepresent invention.

As shown in the FIGS. 1 to 6, the apparatus for manufacturing sterilizednormal saline in accordance with one embodiment of the present inventioncomprises: a container 110 for accommodating the water for manufacturingsterilized normal saline; a spraying unit 120 for spraying thesterilized normal saline to a wound area or inside of a nose; a body 130holding the container 110 and a control circuit; an electrode set 140immersed under the saline solution in the container 110 for generatingoxidants including HOCl by electrolysis; a cover 150 for covering theupper part of the body 130; and a battery 160 of a power supply forsupplying electric power to the electrode set 140.

The container 110 is formed for accommodating saline solution of about0.9% salt concentration by mixing drinking water and salt. In order tomake the about 0.9% salt concentrated saline solution, scale isindicated on the surface of the container 110 for accommodating theexact amount of water (e.g., 50 ml or 100 ml) of which pH is 4.0 to 7.5.As the pH of the accommodated solution is between pH 4.0 to 7.5, most ofthe free chlorines generated therein form HOCl with high sterilizingefficacy.

Also, in order to manufacture medical normal saline with 0.9% saltconcentration, normal saline is poured to the container 110 via theentrance 110 a until the amount of the normal saline in the container110 reaches the indicated scale. Herein, instead of directly pouring thenormal saline into the container 110, after pouring tap water of neutralor subacid into the container 110 firstly, the normal saline can bemanufactured by mixing the water with salt which is released from a saltpackage containing the suitable amount for making water be normalsaline.

The spraying unit 120 includes a plug 121 combined with the entrance 110a of the container 110 for isolating the accommodated solution from theoutside and having a button reciprocally movable, a chamber 124 having achangeable volume in accordance with the reciprocal movement of thebutton so as to suck up the sterilized solution from the container 110,a spring 125 plated with platinum on its surface and installedcompressed in the chamber 124 so that the button can return to itsoriginal position, a spraying pipe which is a passage of the sterilizingwater in the vertical direction for spraying the sterilized normalsaline from inside of the container 110 to the outside, a spray 123 forspraying the sterilized normal saline formed on the outer surface of theplug 121.

Herein, in order to spray the sterilized normal saline to outsidethrough the spraying unit 120, a user presses the button of theapparatus downwardly, then the sterilized normal saline is pumped up tothe spraying unit 120 through the spraying pipe 122 by a instantaneousvolume change of the vacuum chamber 124, and the sterilized normalsaline can be sprayed through the spraying unit 120 in a form of minutewater drops. The normal saline in the container 110 may be supplied to apart of patients or customers in other ways.

The body 130 includes a body case 131 surrounding the container 110 soas to form the exterior of the apparatus 100, a battery cover 132 foropening or closing the battery accommodating part (not shown) foraccommodating two 1.5V batteries for supplying DC current, a switch 133for ordering to supply DC current to the electrode set 140 until thefree chlorines are generated to the concentration of 0.17 ppm to 6 ppm,a first indicator 133 a for indicating the state of operation by colorssuch as red, yellow or green, a circuit accommodation area 135 forinstalling a control circuit such as for supplying DC current to theelectrode set 140, and a bottom area 139 forming the bottom surface ofthe container 110.

When user presses the operation switch 133, DC voltage is supplied tothe electrode unit 140 for a preset time so that the preset amount ofthe free chlorines is generated. Specifically, when the operation switch133 is pressed, DC current is supplied to the electrode set 140 aboutfor 20 seconds so that the free chlorines are generated to be 3 ppm to 4ppm concentration of the normal saline at 20° C. In this regard, in casethat the operation switch 133 is pressed twice continuously, as the freechlorines may be generated more than the intended amount, when anysignal is input by pressing the operation switch 133 more than 2 timeswithin 2 minutes, a message relating thereto is indicated via theindicators 134, 134 a by color signal or by sound signal.

As shown in FIGS. 4 to 8, the electrode set 140 includes a negativeelectrode plate 141, and a positive electrode plate 142 apart from thenegative electrode plate by 2 mm, a support 143 to fix the pair ofelectrodes 141, 142, side support 144 to guide the pair of theelectrodes 141, 142 when the electrodes are being fixed to the support143 and to maintain the interval between the electrodes 141, 142, abottom plate 145 of fixing the support 143, fixing bolts to fix thesupport 143 to the bottom plate 145, and a rubber packing plate 147beneath the bottom plate 145 for preventing solution from permeatinginto the electric components.

As shown in FIG. 5, the support 143 includes concave connection slot1431 for fixing the negative electrode plate 141 and concave connectionslot 1432 for fixing the positive electrode plate 142. As shown in FIG.5, a negative electrode line 161 is connected to the connection slot1431 of the negative electrode plate 141, and the positive electrodeline 162 is connected to the connection slot of the positive electrodeplate 142 inside of the support 143 so that simply inserting theelectrode plates 141, 142 into the slots 1431, 1432 of the support 143can provide an environment of supplying electric power to the electrodeplates 131, 132. Therefore, when a platinum of the electrodes 141, 142is used up, new electrodes can be replaceable by pulling the oldelectrodes 141, 142 from the support 143 and by inserting new electrodes141, 142 into the slots 1431, 1432 of the support 143. Thus, theapparatus 100 can be usable semi-permanently.

Also, the rubber packing plate 147 is attached beneath the bottom plate145 and is placed between the bottom plate 149 and the bottom area 139thereby preventing the normal saline from being leaked to the outside ofthe container 110. Herein, the rubber packing plate 147 may be formed ofring shape instead of plate shape, and thus attached to the circumstanceof the bottom plate 139. The power supply lines 161, 162 are as shown inFIG. 6 connected with the inside of the body case 131 via the bottomplate 145, and thus transport DC current from the batteries 160.

Meanwhile, as shown in FIG. 9 to 11, the electrode set may be formed ofa negative electrode 241 having a plurality of negative rods 241 adivided by vertical slots 241 b, and a positive electrode 242 having aplurality of positive rods 242 a divided by horizontal slots 242 b.Herein, as illustrated in FIG. 11, the negative rods 241 a are arrayedwith the positive rods 242 a at right angle, and the plurality ofdomains facing each other are formed in the overlapped areas 2412 of theelectrodes 241, 242 so that the plurality of electric current paths areformed between the domains of negative electrode 241 and the domains ofpositive domains 242. Therefore, although small amount of voltage issupplied to the plurality of domains which are distributed uniformly,the electric current flows therebetween, thereby extending the life ofbatteries 160. Further, as the electrolysis occurs by supplying smallvoltage to the domains of the electrodes 241, 242, the chemicalreactions by reactants to generate HOCl occurs uniformly distributed.Therefore, as the amount of free chlorines such as HOCl is generatedconstantly and uniformly distributed compared with the case using flatshaped electrodes, the concentration of the free chlorines can beprecisely controlled.

Herein, in order to prevent that the electrolysis occurs more vigorouslyalong the circumstance area 241 c, 242 c of the electrode 241, 242 thanat the plurality of domains 2412, coating layer is formed on thecircumference area 241 c, 242 c except the area surrounded by x, y ofFIG. 9. Also, the width d1 of the negative rod 241 a and the positiverod 242 a is smaller than the width d2 of the negative slot 241 b andthe positive slot 242 b. Thus, the size of the domains 2412 becomesshorter than the distances between the domains 2412 whereby thereactants to generate HOCl can be uniformly and effectively distributedaround current paths between the domains 2412 so that HOCl can beconstantly generated by the adequate and continuous supply of thereactants. Therefore, the precise control of the HOCl is more easilyachievable.

On the other hand, the electrode set having the plurality of domainsfacing each other may be formed as shown in FIG. 12. That is, theelectrode set of FIG. 12 includes a negative electrode plate 341 havinga plurality of negative projections 341 a protruded thereon, a positiveelectrode plate 342 having a plurality of positive projections 342 a theprotruded thereon each of which faces each of the negative projections341 a, wherein the top areas A of both the plurality of negativeprojections 341 a and the plurality of positive projections 342 a formthe plurality of domains facing each other. Similarly, the negativeelectrode plate 341 and the positive electrode plate 342 are fixed tothe support 143 with maintaining a constant interval d4, and thus thenegative projections 341 a and the positive projections 342 a on thefacing surfaces B are separated each other by the interval d3, wherebyelectric charges are concentrated on the top areas A of the projections341 a, 342 a. Therefore, although small voltage is supplied to theelectrode 341, 342, the electric current can flow between the domains Afacing each other of the electrodes 341, 342.

A platinum is plated on the surface of the electrodes 141, 142, 241,242, 341, 342 thereby inducing the acute electrolysis.

The control circuit installed in the circuit accommodation area 135controls to supply DC current to the electrodes 141, 142, 241, 242, 341,342 for a preset time in accordance with the input of switch 133, andcontrols the indicators 133 a, 134, 134 a to indicate the operationstate, and controls to convert the direction of the DC current in every3 seconds to 7 seconds which is supplied to the electrodes 141, 142,241, 242, 341, 342 thereby helping to precisely control theconcentration of the free chlorines. Also, the control circuit includesa part for constantly supplying DC current to the electrodes 141, 142,241, 242, 341, 342. Therefore, although a voltage of the new batteries160 is initially 3.3V, only 2.2V to 2.5V is applied to the electrodes141, 142, 241, 242, 341, 342 by the control circuit thereby making DCcurrent between the electrodes 141, 142, 241, 242, 341, 342 as lower aspossible. Herein, as the flat electrodes 141, 142 require higher voltageso as to make DC current flow therebetween, 2.4V is supplied to theelectrodes 141, 142. On the other hand, as the electrodes 241, 242, 341,342 require lower voltage so as to make DC current flow therebetween,2.2V is supplied to the electrodes 241, 242, 341, 342.

Concretely, the circuit shown in FIG. 15 is to constantly maintain theDC current between the electrodes 141, 142, 241, 242, 341, 342 in spiteof the consumption of batteries 160 and of the differences of the saltconcentration. Also, the control circuit periodically converts thedirection of DC current between the electrodes 141, 142, 241, 242, 341,342, each of the initial negative electrodes 141, 241, 341 plays a rolein the positive electrode, and similarly, each of the initial positiveelectrodes 142, 242, 342 also plays a role in the negative electrode,thereby preventing products of the chemical reaction from sticking ontothe each of the electrodes 141, 142, 241, 242, 341, 342. In order torealize this operation, two pairs of transistors TR1, TR2, TR3, TR4 as aswitch element are connected in parallel with each other, and theelectrodes 141, 142, 241, 242, 341, 342 are placed between points 181,182 which are located between the each pair of the transistors. Thus,when TR1 and TR4 are ON and when TR2 and TR3 are OFF, the DC currentflows from the first point 181 to the second point 182, while TR1 andTR4 are OFF and when TR2 and TR3 are ON, the DC current flows from thesecond point 182 to the first point 181.

Herein, the saline solution between the electrodes 141, 142, 241, 242,341, 342 plays a role in the resistance 111 a of the circuit shown inFIG. 15. Thus, although a scale is indicated on the container 110 foraccommodating the constant amount of saline solution, the saltconcentration of the saline solution may be different by thecarelessness of a user. Accordingly, the resistance 111 a may bedifferent, and the DC current also may be different from the presetvalue. In order to compensate the difference of DC current, actual DCcurrent is sensed by a resistance R1 which is serially connected fromthe batteries 160.

For example, when TR1 and TR4 are ON and when TR2 and TR3 are OFF andthus DC current flows from the first point 181 to the second point 182,the DC current on the resistance R1 is sensed as a pulse form 66.Herein, when the DC current is higher than the preset value, if the basecurrent IB is minutely raised, as a electric power is more consumed atTR1 and TR4, the width of the pulse 66 becomes smaller thereby loweringthe voltage over the saline solution, and therefore, the DC currentbetween the electrodes 141, 142, 241, 242, 341, 342 becomes smaller sothat the targeted preset value of DC current can be applied to theelectrodes 141, 142, 241, 242, 341, 342. Similarly, when the DC currentis lower than the preset value, if the base current IB is minutelylowered, as a electric power is less consumed at TR1 and TR4, the widthof the pulse 66 becomes bigger thereby raising the voltage over thesaline solution, and therefore, the DC current between the electrodes141, 142, 241, 242, 341, 342 becomes bigger so that the targeted presetvalue of DC current can be applied to the electrodes 141, 142, 241, 242,341, 342. That is, the difference of the DC current in accordance withthe differences of salt concentration can be compensated constantly bythat IC 173 controls the base current I_(B) to the transistors TR1, TR2,TR3, TR4 and thus controls the pulse width of DC current flowing betweenelectrodes.

Similar operation principle is also applied in compensating thedifferences of DC current in accordance with the voltage drop due to theuse of the batteries 160. Although the initial voltage of the batteries160 is 3.3V, the voltage drops to 2.3V in accordance with a use of thebatteries 160. Therefore, when DC current is applied to the electrodes141, 142, 241, 242, 341, 342 for an initial preset time, as the voltagedrops, the normal saline may be excessively sterilized when 3.3V isinitially supplied thereto while the normal saline may be lesssterilized when 2.3V is later supplied thereto. In order to solve thisproblem, the DC current is measured at the resistance R1 in-real time(herein, the DC current at R1 is the same at the electrodes 141, 142,241, 242, 341, 342 because the resistance R1 is serially connected withthe electrodes 141, 142, 241, 242, 341, 342). Thereafter, when the DCcurrent at R1 is higher than the preset value to be supplied to theelectrodes 141, 142, 241, 242, 341, 342, the minutely increased basecurrent IB is applied to the transistors of which state is ON so thatthe voltage drop is induced to occur at the transistors, whereby the DCcurrent can be maintained constantly in spite of the voltage change fromthe batteries 160.

From these constructions, although the salt concentration and thebattery voltage may be inconsistent, the DC current applied to theelectrodes can be constantly maintained, whereby the sterilized normalsaline with the constantly lowly concentrated free chlorine such as HOClcan be reliably and stably obtained.

The cover 150 plays a role in covering the upper part of the body 130and in accommodating the salt package of which amount is to weighed tomake normal saline of about 0.9% salt concentration in the container110. Thus, a user conveniently carries the required salt within theaccommodating part 150 a together with the apparatus 100. A lid 152opens or closes the accommodating part 150 a by combining with thecombining part 151.

The batteries 160 as a power supply is formed of a pair of 1.5V ratedvoltage batteries. The batteries 160 supply 30 mA to 200 mA DC currentto the electrodes 141, 142, 241, 242, 341, 342 via the power supplylines 161, 162 by the control circuit for the preset time. Without auser's control, the control circuit controls to periodically convert thedirection of DC current in every 3 seconds to 7 seconds.

When the DC voltage is applied to the electrodes from the batteries 160,in accordance with the above procedures (1) to (5), the apparatus 100manufactures the sterilized normal saline with 0.17 ppm to 6 ppm of lowconcentration of the residual concentration, wherein most of the freechlorines are formed of HOCl.

Hereinafter, the principle of the apparatus for manufacturing sterilizednormal saline in accordance with the present of the invention will bedescribed.

When a user manufactures sterilized normal saline using the apparatus100, the user inputs subacid or neutral tap water and the salt in thepackage into the container 110 and mix them to make about 0.9% salinesolution. Thereafter, the power is supplied via the slots 1431, 1432 tothe electrodes 341, 342. Herein, a current is measured at the resistanceR1 which is serially connected with the batteries 160, and aftercomparing the measured current with the preset current, the DC currentto the electrodes 341, 342 is compensated by controlling the basecurrent IB to the transistors by the difference between the measuredcurrent and the preset current.

Herein, the electric charges supplied to the electrodes 341, 342 areconcentrated on the projections 341 a, 342 a facing each other. Thus,the electrolysis occurs at the plurality of current paths between theprojections 341 a,342 a facing each other whereby the reactants of theelectrolysis can be effectively reacted. The oxidants in the normalsaline such as ozone, H₂O₂, HOCl, OCl⁻, OH radicals generated by theelectrolysis sterilize and kill germs, protein, fungi, bacteria, etc.within a short time. Especially, as the normal saline is neutral orsubacid, most of the low concentrated free chlorines are formed of HOClwhich has strong sterilizing efficacy thereby enabling to apply to themedical purpose and achieving highly improved sterilizing effect.

The apparatus 100 necessarily requires the only electrode set 241, 242,341, 342 with plural domains A, 2412 facing each other, and thus can beconstructed as a small portable one enough for a user to carryconveniently.

Hereinafter, the example of the sterilized normal saline in accordancewith a first embodiment of the present invention will be described.

1^(st) Embodiment

The electrodes 141,142 are formed of a plate-shape having its surfacearea of 1225 mm² which are plated with platinum and arrayed apart by aninterval d of 2 mm from each other and immersed in the normal saline of100 ml having pH 6.45±0.2. Also, DC current is supplied to theelectrodes 141, 142 for 20 seconds with periodically converting thedirection of the DC current in every 5 seconds. The concentration of thefree chlorines was measured for 5 times with respect to the voltagechange as shown in the following Table-2.

TABLE 2 Voltage DC Average Standard Deviation Supply CurrentConcentration of Concentration No. (V_(DC)) (mA) of Free chlorines ofFree chlorines 1-1 2.2 Not flow — — 1-2 2.4 80 1.20 0.45 1-3 2.7 1602.10 0.71 1-4 3.3 200 3.04 0.81 1-5 3.5 350 5.81 1.24 1-6 4.5 520 7.141.72

As shown in the experimental result Table-2, DC current cannot flowbetween the electrodes 141, 142 when DC 2.2V is supplied to theelectrodes 141, 142, while average DC current of 80 mA flows between theelectrodes 141, 142 when DC 2.4V is supplied thereto thereby generatingvery low concentrated (i.e., average 1.2 ppm) free chlorines. Average DC200 mA flows between the electrodes 141, 142 in accordance with thesupply of DC 3.3V and thus generates the still low concentrated (i.e.,average 3.04 ppm) free chlorines. However, when DC 3.5V is supplied tothe electrodes 141, 142, average DC current of 350 mA which is suddenlyraised flows between the electrodes 141, 142 thereby generating the 5.81ppm concentrated free chlorines. That is, in case that DC 3.5V slightlyhigher than DC 3.3V is supplied to electrodes 141, 142, the DC currentbetween the electrodes 141, 142 is sharply. Although this concentrationof 5.81 ppm is slightly less than 6 ppm, in view that the standarddeviation is relatively higher value of 1.24, it can be concluded thatthe supply of DC 3.5V cannot guarantee the free chlorines less than 6ppm.

2^(nd) Embodiment

The width d1 of rods 241 a, 242 a of the electrodes 241,242 are formedof 0.7 mm, and the width d2 of slots 241 b, 242 b of the electrodes241,242 are formed of 1.3 mm. The electrodes 241,242 are arrayed so thatthe negative rods 241 a are positioned at right angle with the positiverods 242 a. Each of the center areas including the domains (i.e., wholesurface area of the rods and slots) surrounded by the x, y of FIG. 9 is841 mm² respectively and are plated by platinum, and the other surfaceareas (i.e., circumstance area 241 b, 242 b excluding the center area)of the electrodes 241, 242 are coated by insulating layer so as toprevent the electrolysis from occurring at the circumference areas 241b, 242 b. The electrodes 241, 242 are arrayed apart by 2 mm from eachother and immersed in the normal saline of 50 ml having pH 6.37±0.2.Also, DC current is supplied to the electrodes 241, 242 for 20 secondswith periodically converting the direction of the DC current in every 5seconds. The concentration of the free chlorines was measured for 5times with respect to the voltage change as shown in the followingTable-3.

TABLE 3 Average Voltage DC Concentration Standard Deviation SupplyCurrent of Free chlorines of Concentration No. (V_(DC)) (mA) (ppm) ofFree chlorines 2-1 2.1 Not flow — — 2-2 2.2 40 1.01 0.21 2-3 2.4 50 1.620.31 2-4 2.6 65 2.83 0.25 2-5 2.7 75 3.04 0.33 2-6 2.9 85 3.20 0.30 2-73.0 100 3.57 0.66 2-8 3.2 120 4.30 0.78 2-9 3.4 160 5.57 1.51

As shown in the experimental result Table-3, DC current cannot flowbetween the electrodes 241, 242 when DC 2.1V is supplied to theelectrodes 241, 242, while average DC current of 40 mA flows between theelectrodes 241, 242 when DC 2.3V is supplied thereto thereby generatingvery low concentrated (i.e., average 1.01 ppm) free chlorines. AverageDC 120 mA flows between the electrodes 241, 242 in accordance with thesupply of DC 3.2V and thus generates the still low concentrated (i.e.,average 4.3 ppm) free chlorines. However, when DC 3.3V is supplied tothe electrodes 241, 242, average DC current of 160 mA which is suddenlyraised flows between the electrodes 241, 242 thereby generating the 5.57ppm concentrated free chlorines. That is, in case that DC 3.4V slightlyhigher than DC 3.2V is supplied to electrodes 241, 242, the DC currentbetween the electrodes 241, 242 is sharply increased in accordance withButler-Volmer Equation. Although this concentration of 5.57 ppm isslightly less than 6 ppm, as the standard deviation is relatively highervalue of 1.51, it can be concluded that the supply of DC 3.5V cannotguarantee the free chlorines less than 6 ppm.

Although not shown in the above table-3, regarding the small amount ofless than 150 ml normal saline, it is confirmed by the experiment thatthe amount of the free chlorines generated by the electrolysis increasesin the same way to the above result except for longer operation time.

3^(rd) Embodiment

The width d1 of rods 241 a, 242 a of the electrodes 241,242 are formedof 0.3 mm, and the width d2 of slots 241 b, 242 b of the electrodes241,242 are formed of 0.8 mm. The electrodes 241,242 are arrayed so thatthe negative rods 241 a are positioned at right angle with the positiverods 242 a. Each of the center areas including the domains (i.e., wholesurface area of the rods and slots) surrounded by the x, y of FIG. 9 is841 mm² respectively and are plated by platinum, and the other surfaceareas (i.e., circumstance area 241 b, 242 b excluding the domain area)of the electrodes 241, 242 are coated by insulating layer so as toprevent the electrolysis from occurring at the circumference areas 241b, 242 b. The electrodes 241, 242 are arrayed apart by 2 mm from eachother and immersed in the normal saline of 50 ml having pH 6.45±0.2.Also, DC 2.7V is supplied to the electrodes 241, 242 for 20 seconds withchanging the conversion period of the direction of the DC current. Theconcentration of the free chlorines was measured for 5 times withrespect to the conversion period change as shown in the followingTable-4.

TABLE 4 Current Direction Conversion DC Average Standard DeviationPeriod Current Concentration of Concentration No. (second) (mA) of Freechlorines of Free chlorines 3-1 1 Cannot 1.61 0.47 measure 3-2 2 Cannot2.16 0.51 measure 3-3 5 70 2.97 0.40 3-4 7 70 3.40 0.89 3-5 12 70 3.730.51 3-6 15 70 4.11 0.61 3-7 18 70 4.63 0.66 3-8 19 70 4.80 0.78 3-9 Notchange 70 5.53 0.95

The experimental result of Table-4 shows that the average concentrationof the free chlorines becomes smaller as the conversion period becomesshorter. On the other hand, in case that the direction of the DC currentis not changed for 20 seconds, it shows that the possibility ofexceeding 6 ppm of the concentration thereof increases. However, in casethat the conversion period becomes too short, the DC current cannot bemeasured but the deviation of the concentration thereof shows a littlebit high. Therefore, it was concluded that 5 second period is the mostsuitable for maintaining the concentration below 6 ppm and for reducingthe deviations of the concentration. That is, comparing the case of 5second period with the case of not having the conversion period, theconcentration of the free chlorines and the deviation thereof in theformer case with the conversion period becomes a half than that in thelater case without the conversion period.

Meanwhile, although the data is not shown in the above Table-4 for thesimple flat electrodes 141, 142, it has been confirmed that a trend forthe flat electrodes 141, 142 is similar with the above case. That is,the case of applying 5 second period to convert the direction of the DCcurrent generates about the half amount of concentration of the freechlorines and shows the half deviations of the concentrationdistribution compared with the case of not applying the conversionperiod.

4^(th) Embodiment

The experiments is to performed what effect is influenced by the changesof the width of the rods 241 a, 242 a and the width of the slots 241 b,242 b on the concentration of the free chlorines. Similarly with the2^(nd) embodiment, the electrodes 241,242 are arrayed so that thenegative rods 241 a are positioned at right angle with the positive rods242 a. Each of the center areas including the domains (i.e., wholesurface area of the rods and slots) surrounded by the x, y of FIG. 9 is841 mm² respectively and are plated by platinum, and the other surfaceareas (i.e., circumstance area 241 b, 242 b excluding the center area)of the electrodes 241, 242 are coated by insulating layer so as toprevent the electrolysis from occurring at the circumference areas 241b, 242 b. The electrodes 241, 242 are arrayed apart by 2 mm from eachother and immersed in the normal saline of 50 ml having pH 6.45±0.2.Also, DC 3.0V is supplied to the electrodes 241, 242 for 20 seconds withchanging the conversion period of the direction of the DC current. Theconcentration of the free chlorines was measured for 5 times withrespect to the conversion period change as shown in the followingTable-5.

TABLE 5 Width Width Average Standard of of DC Concentration Deviation ofRod Slot Current of Concentration of No. (mm) (mm) (mA) Free chlorinesFree chlorines 4-1 0.7 1.3 100 3.57 0.66 4-2 0.3 0.8 80 3.01 0.49 4-30.5 1.0 85 3.24 0.61 4-4 1.0 1.0 110 4.07 1.11

The experimental result of Table-5 shows that the DC current becomessmaller as the area of domains 2412 facing each other becomes smallerfor a unit area and distributed widely for a unit area. Therefore, itcan be concluded that it is more efficient for precisely controlling theconcentration of the free chlorines to uniformly distribute theplurality of domains 2412 which are formed as small as possible.

The Sterilizing Effect of Normal Saline of the Present Invention

The sterilized normal saline of 35 ml having 0.85% salt concentration ismanufactured by electrolysis for 20 seconds, and then has been appliedonly 30 seconds to germs of Staphylococcus aureus MRSA for Time KillTest by Polymer Solutions Incorporated in U.S.A. The test result is asfollows.

TABLE 6 Initial CFU/ml contents 1^(st) Test 2^(nd) Test 2.8 × 10⁶ LivingCFL Less than 5 Less than 5 2.8 × 10⁶ Killing percentage 99.9998%99.9998% 2.8 × 10⁶ Log₁₀ (reduction rate) 5.75 5.75

Herein, CFL means the numbers of living germs, and CFU/mL means thenumber of living germs population. The above test result shows that thecontact with the sterilized normal saline only for 30 seconds makes99.9998% of the germs named of Staphylococcus aureus MRSA killed.

Also, the sterilized normal saline of 50 ml having 0.80% saltconcentration is manufactured by electrolysis for 20 seconds and thushaving 3 ppm to 4 ppm concentrated free chlorines has been applied tothe following germs for Time Kill Test. The test result is as follows.

Initial Count Result Log₁₀ Organism (CFU/mL) IC Log₁₀ Time Point Rep.(CFU/mL) Reduction % Reduction A 1.00E+07 7.00 30 sec. 1 3.00E+04 2.5299.700000% Aspergillus fumigatus 2 3.00E+04 2.52 99.700000% B 9.10E+077.96 30 sec. 1 3.00E+04 3.48 99.967033% Rhizopus oryzae 2 3.00E+04 3.4899.967033% C 7.80E+05 5.89 30 sec. 1 5.60E+03 2.14 99.282051%Haemophilus influenae 2 4.50E+03 2.24 99.423077% D 8.60E+05 5.93 30 sec.1 4.30E+03 2.30 99.500000% Strptococcus pneumoniae 2 5.10E+03 2.2399.406977% E 9.80E+05 5.99 30 sec. 1 5.20E+03 2.28 99.469388%Strptococcus pyogenes 2 5.70E+03 2.24 99.418367% F 1.60E+06 6.20 30 sec.1 7.20E+04 1.35 95.500000% Candida albicans 2 6.30E+04 1.40 96.062500% G7.40E+07 7.87 30 sec. 1 4.00E+01 6.27 99.999946% Klebsiella pneumoniae 21.00E+01 6.87 99.999986% H 5.00E+07 7.70 30 sec. 1 3.00E+04 3.2299.940000% Staphylococcus aureus MRSA 2 3.00E+04 3.22 99.940000%

The A, B, C items of the listed germs indicate the fungus-kind germs,and the C, D, E, F, H items thereof indicate the bacteria-kind germs.The above test result shows that contact of the germs for 30 secondswith the sterilized normal saline of the present invention realizes the95.5% to 99.9% killing effect.

INDUSTRIAL APPLICABILITY

The medical sterilized normal saline of the present invention can besupplied to cure an allergy, rhinitis by transforming protein that iscause of allergy and can be sprayed to a throat for sterilizing insideof mouth and throat directly after being manufactured. Also, thesterilized normal saline can cure athletes' foot, an inflammation and awounded area by spraying right after being manufactured. Further, thesterilized normal solution may be used to sterilize scurfy germs ofhair, and may be used to kill germs or microbes in the shoes. Moreover,the sterilized normal saline may be applied to womb or vagina for curingcontamination of HPV (human papillomavirus), teeth and germs, and mouthfor gargling.

More concretely, the contact lens cleaning module 420 shown in FIGS. 17and 18 may be used to fix to the entrance 110 a of the container 110 forcleaning contact lens. That is, the contact lens module 420 includes aplug 421 for being fixed to the entrance 110 a, an extending member 422extended from the plug 421, a lens accommodating chamber 423 located atthe extending member 422 so that lenses accommodated in the chamber 423may be positioned in the sterilized normal saline. Herein, the wall ofthe lens accommodating chamber 423 is formed of wire so that thesterilized normal saline can enter the chamber 423 and sterilize theeach of lenses in the chamber 423. Therefore, the oxidants including thefree chlorines generated by the electrolysis sterilize or kill thegerms, bacteria on the contact lenses while the hydrogen peroxideremoves the protein on the surface of the contact lens.

On the other hand, the supplying module 500 shown in FIGS. 19 and 20 canbe used for supplying sterilized normal saline into deep place by fixingthe inlet 110 a of the container 110. Herein, the supplying module 500comprises a plug to be fixed to the entrance 110 a so as to interceptthe air flow, an air tube 510 extended from the plug to over the surfaceof the normal saline when the container 110 is reversed, a fluid tube520 extended from the lid to outside so as to make the sterilized normalsaline flow out. When the supplying module is fixed to the inlet 110 aand the apparatus 100 is reversed, although any driving means to exhaustor spray the normal saline does not included, in condition that the headof a user is located below feet, air is induced into the container 110through the air tube 510, and thus, the sterilized normal saline iscapable of coming from the container into the inside of nose or lungthrough the fluid tube 520. In order to prevent the fluid tube 520 fromdamaging the organs of human body, protect socket 521 having roundedshape is attached at the end of the fluid tube 520. Also, so as toprompt the exhausting of the sterilized normal saline from thecontainer, air balloon is attached at the end of the air tube 510.

From this construction with a long tube 520, the medical sterilizednormal saline can be supplied to a deep womb, a deep throat or a deeplung easily. Also, at the end of the tube 520, a mirror or lens may beattached so that a operator easily check whether the normal saline isbeing properly supplied.

Further, a holder shaped of a reversed cup may be attached at the end ofthe fluid tube 520 of the supplying module 500, and thus, it is possibleto continuously supply the oxidants in the sterilized normal solution onskin for the time being such as 30 seconds to even 2 minutes. Herein, inorder to make contact the newly generated oxidants with a user's skin,with constantly inducing electrolysis by continuously supplying lowcurrent to the electrode set 140, it is also possible to continuouslysupplying fresh oxidants into the holder from the container 110 byletting the sterilized normal saline leak via a small hole. Also, asealing rubber packing is formed on the circumstances of the holder soas to prevent the sterilized normal saline from being leaked between theuser's skin and inside of the holder.

On the other hand, when user wishes to supply the sterilized normalsaline to the womb or vagina, it is desirable to use womb supplyingmodule including a supply socket at the end of the supply tube whereinthe supply socket has holes in the radial direction which allowssterilized normal saline to be evenly supplied into the womb. Althoughnot shown in the figures, at least one spherical mirror is attached atthe supply socket, and thus, the user can insert the supplying modulefor herself likewise the principle of endoscopy.

As the present invention may be embodied in several forms withoutdeparting from the spirit or essential characteristics thereof, itshould also be understood that the above-described embodiments are notlimited by any of the details of the foregoing description, unlessotherwise specified, but rather should be construed broadly within itsspirit and scope as defined in the appended claims, and therefore allchanges and modifications that fall within the metes and bounds of theclaims, or equivalence of such metes and bounds are therefore intendedto be embraced by the appended claims.

1. A method for manufacturing sterilized normal saline for medicalpurpose with effective sterilizing efficacy comprising: a step ofdisposing at least one electrode set immersed in saline solution of pH4.0 to pH 7.5 including a pair of electrodes with flat surface separatedfrom each other by an interval between 1 mm and 3 mm, the flat surfacesof the electrodes facing each other; a step of supplying 30 mA to 200 mAdirect current to the electrodes by applying 2.4V to 3.3V DC power tothe electrodes; wherein free chlorine is generated as havingconcentration range between 0.17 ppm and 6 ppm from electrolysis betweenelectrodes.
 2. The method for manufacturing sterilized normal saline formedical purpose as claimed in claim 1, wherein the saline solution isnormal saline.
 3. The method for manufacturing sterilized normal salinefor medical purpose as claimed in claim 2, wherein the direction of thedirect current is reversed at least one time during the electrolysis. 4.The method for manufacturing sterilized normal saline for medicalpurpose as claimed in claim 3, wherein the direction of the directcurrent is reversed every one second to every 20 seconds.
 5. The methodfor manufacturing sterilized normal saline for medical purpose asclaimed in claim 1, wherein the amount of normal saline is 10 Ml to 100Ml and the normal saline is directly manufactured by a portableapparatus.
 6. The method for manufacturing sterilized normal saline formedical purpose with sterilizing efficacy comprising: a step ofdisposing at least one electrode set immersed in saline solution of pH4.0 to pH 7.5 including a pair of electrodes with a plurality of domainsseparated from one another on the facing surface of each electrode, theplurality of domains on the facing surface of the one electrode beingfaced to the plurality of domains on the facing surface of the otherelectrode with an internal between 1 mm and 3 mm; and a step ofsupplying 30 mA to 180 mA of DC to the electrodes by applying 2.2V to3.2V DC power to the electrodes, wherein the area of the plural domainsof each of the electrodes covers 4% to 25% of the total area of thefacing surface of each electrode and the free chlorine is generated ashaving concentration range between 0.17 ppm to 6 ppm during theelectrolysis.
 7. The method for manufacturing sterilized normal salinefor medical purpose as claimed in claim 6, wherein the saline solutionis normal saline.
 8. The method for manufacturing sterilized normalsaline for medical purpose as claimed in claim 7 further comprising: astep of reversing the direction of the direct current flowing throughthe electrodes at least one time during the electrolysis.
 9. The methodas claimed in claim 6, wherein the electrode set comprises: aplate-shaped positive electrode having a plurality of positive rodsdivided by a plurality of positive slots in one direction which areparallel with one another; and a plate-shaped negative electrode havinga plurality of negative rods divided by a plurality of negative slots inone direction which are parallel with one another wherein the pluralityof domains are formed by the areas overlapped in the perpendiculardirection to the surface of the electrodes when the positive electrodeand the negative electrode are disposed in parallel so that the positiverods are not disposed to be parallel with the negative rods.
 10. Themethod for manufacturing sterilized normal saline for medical purpose asclaimed in claim 9, wherein the positive rods and the negative rods aredisposed at right angles to each other.
 11. The method for manufacturingsterilized normal saline as claimed in claim 9, wherein the width of thepositive rods is narrower than the width of the positive slots, and thewidth of the negative rods is narrower than the width of the negativeslots.
 12. The method for manufacturing sterilized normal saline formedical purpose as claimed in claim 6, wherein the electrodes comprises,a plurality of positive projections formed protrudedly on the positiveelectrodes; and a plurality of negative projections formed protrudedlyon the facing surface of the negative electrodes; wherein the pluralityof domains are formed by areas of the end surface of the positiveprojections and the negative projections facing each other.
 13. Themethod for manufacturing sterilized normal saline for medical purpose asclaimed in claim 6, wherein the direction of the direct current flowingthrough the electrodes is reversed every one second to every 20 seconds.14. The method for manufacturing sterilized normal saline for medicalpurpose as claimed in claim 6, wherein the amount of the normal salineis 10 Ml to 100 Ml and the normal saline solution is directlymanufactured by a portable apparatus.
 15. The method for manufacturingsterilized normal saline for medical purpose as claimed in claim 6,wherein the amount of the normal saline is 10 Ml to 100 Ml and thedirect current is supplied to the electrodes for 10 to 60 seconds. 16.The method for manufacturing sterilized normal saline for medicalpurpose with sterilizing efficacy comprising: a step of disposing atleast one electrode set immersed in saline solution of pH 4.0 to pH 7.5including a pair of electrodes with a plurality of domains separatedfrom one another on the facing surface of the each of the electrodes,the plurality of domains on the facing surface of the one electrodebeing faced to the plurality of domains on the facing surface of theother electrode with an interval between 1 mm to 3 mm, wherein the areaof the plurality of domains of each of the electrode covers 4% to 25% ofthe total area of the facing surface of each of the electrode; and astep of electrolysis by supplying DC to the electrodes;
 17. The methodas claimed in claim 16 further comprising: a step of reversing thedirection of the direct current flowing through the electrodes at leastone time during the electrolysis.
 18. The sterilized normal saline formedical purpose manufactured by the method in accordance with claim 1,wherein the manufactured normal saline is used for at least one ofspraying to the inside of a nose, spraying to the throat, gargling theinside of a mouth, spraying to teeth or gums, disinfecting body partsafter operations, spraying to the skin, spraying to the hair, sprayingto bronchi or lungs, spraying the inside of the womb for disinfectionand spraying to the athlete's foot.
 19. The sterilized normal saline formedical purpose manufactured by the method in accordance with claim 6,wherein the manufactured normal saline is used for at least one ofspraying to the inside of a nose, spraying to the throat, gargling theinside of a mouth, spraying to teeth or gums, disinfecting body partsafter operations, spraying to the skin, spraying to the hair, sprayingto bronchi or lungs, spraying the inside of the womb for disinfectionand spraying to the athlete's foot.
 20. The medical sterilized normalsaline manufactured by the method in accordance with claim 16, whereinthe manufactured normal saline is used for at least one of spraying tothe inside of a nose, spraying to the throat, gargling the inside of amouth, spraying to teeth or gums, disinfecting body parts afteroperations, spraying to the skin, spraying to the hair, spraying tobronchi or lungs, spraying the inside of the womb for disinfection andspraying to the athlete's foot.
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